Team:AshesiGhana/Design
BBa_J23100
Constitutive promoter
It is the DNA sequence that allows RNA polymerase to bind in order to initiate the transcription of DNA to mRNA.
BBa_K1071003
Light-inducible promoter
It is a promoter that was derived from gene fcpB. It initiates transcription only in the presence of light.
BBa_K2348001
asr promoter
It is an acid-induced promoter that initiates transcription at a pH of 4.8.
BBa_K234800
alx promoter
It is an alkaline induced promoter that is activated and initiates transcription at a pH of 8.5.
BBa_J61100
RBS
The ribosome binding site is a nucleotide sequence to which the ribosome can bind to begin the translation of mRNA.
BBa_B0010
Terminator
It is the nucleotide sequence that signals the end of a gene and ends transcription.
BBa_K3368000
PETase
It is the enzyme that breaks down polyethylene terephthalate (PET) into mono-2-hydroxyethyl terephthalate (MHET) and terephthalate (TPA).
BBa_K3368001
MHETase
It is the enzyme that is responsible for breaking down MHET into TPA and ethylene glycol.
BBa_K3368002
UreA
The first coding sequence in the UreABC operon. It codes for the first subunit of the enzyme urease that is involved in the hydrolysis of urea into ammonium and carbonate ions.
BBa_K3368003
UreB
The second coding sequence in the UreABC operon. It codes for the second subunit of the enzyme urease that is involved in the hydrolysis of urea into ammonium and carbonate ions.
BBa_K3368004
UreC
The third coding sequence in the UreABC operon. It codes for the third subunit of the enzyme urease that is involved in the hydrolysis of urea into ammonium and carbonate ions.
BBa_K3000026
ompT tag
It is a secretion tag that is transports proteins from the interior to the exterior of the cell.
These five recombinant plasmids included the following inserts:
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A plastic biodegradation insert with a constitutive promoter (BBa_K3368006)
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A plastic biodegradation insert with a high pH inducible promoter (BBa_K3368007)
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A biocementation insert with a constitutive promoter (BBa_K3368008)
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A biocementation insert with a low pH inducible promoter (BBa_K3368010)
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A biocementation insert with a light-inducible promoter (BBa_K3368009)
This section contains the plasmid we used and the five recombinant that were obtained in silico using the SnapGene Software
Fig. Diagram of the non- cloned plasmid (pSB1C3) which was used as the vector for cloning
Fig. Diagram of the Cloned plasmid (pSB1C3) with plastic biodegradation insert with a constitutive promoter
Fig. Diagram of the Cloned plasmid (pSB1C3) with plastic biodegradation insert with a High pH-Inducible promoter
Fig. Diagram of the Cloned plasmid (pSB1C3) with biocementation insert with a constitutive promoter
Fig. Diagram of the Cloned plasmid (pSB1C3) with biocementation insert with a high pH-Inducible promoter
Fig. Diagram of the Cloned plasmid (pSB1C3) with biocementation insert with a light-Inducible promote