Overview

In the degradation part, we redesigned the MlrA enzyme of 2014_Peking ( BBa_K1378001 ) and characterized its degradation rate. We have also characterized our new part, MlrA enzyme from Novosphingobium sp. THN1. The results of the two parts are compared. It is indicated that both of the MlrA enzymes can degrade MC-LR with favorable efficiently.

In order to explore the properties of purified MlrA enzyme, we designed another two parts to purify the MlrA protein. We used the T7 promoter plus 6xHis tag or MBP tag to facilitate the expression and purification. Sequences encoding fusion proteins 6xHis-mlrA ( BBa_K3699009 ) and 6xHis-MBP-mlrA ( BBa_K3699010 ) are improved parts of BBa_K1378001.

We hope that the reproduction of cyanophages is under our control. In the control part, the “tail” protein with UAG inserted and the orthogonal translation system are created. The introduction of stop codon caused the early termination of protein translation. If the stop codon was introduced into the cyanophage genome, it will become a virus that cannot release or infect cyanobacteria, thus preventing the escape of recombinant phage. The orthogonal translation system has been used in vaccine safety design. The Pyrrolysyl tRNA synthetase/tRNA (pylrs/tRNA) from Methanosarcina mazei is used to achieve the anti-escape design of cyanophages.

Basic Parts

Composite Parts

Characterized Parts

Improved Parts

Reference

[1] Bourne D G . Enzymatic pathway for the bacterial degradation of the cyanobacterial cyclic peptide toxin microcystin LR.[J]. Applied & Environmental Microbiology, 1996, 62.

[2] Daichi M , Shigeko K , Yoshihiko S , et al. Transcriptome Analysis of a Bloom-Forming Cyanobacterium Microcystis aeruginosa during Ma-LMM01 Phage Infection[J]. Frontiers in Microbiology, 2018, 9:2-.