Team:Edinburgh/Contribution


Team Edinburgh Finding NEMO

Testing the BBa_z0251 promoter



The development of a cell-free modular biosensor has multiple advantages. Besides the obvious ones such as: no GMO's, direct contact of the analyte and faster synthesis time, there is another important one, modularity. It allowed us to have a wide range of choice when deciding what parts to choose. There are multiple promoters, terminators and transcription factors besides the ones described in the iGEM registry. However, we decided to use a promoter from the iGEM registry to build our construct. There was no experience description for the BBa_Z0251 T7 promoter and because iGEM is also about creating and documenting DNA parts we chose it.

We tested the promoter in the BBa_3K380500 construct. It did work as expected, however, it wasn't compared to any other T7 equivalent promoters, due to limited access to the lab during the pandemic. We documented our findings on the experience page as well as exhibiting some results that we obtained using the BBa_Z0251 promoter part.

COLLABORATIVE MANUAL AND OPEN DATA(file repository)



Another that could constitute a contribution to the iGEM community is our Collaborative Manual that will be passed to the next wave of iGEMers and the next Edinburgh's iGEM team

The manual will be still active after the wiki freeze and will be completed and edited at the end of the competition

We are giving the opportunity the other iGEM teams to send us part of their wiki that is related to detection systems/biosensors after the wiki freeze since some teams that were highly interested found it difficult to participate to the initiative due to lab work and other activities

The Biosensor Manual is not the only document that is publicly available, part of our documentation had been publicly available for months and we are planning to make our repository open so that future iGEM team can have a look at what we have done, look at our data and reuse documents and presentations
Currently only part of our repository is available to the public since there are still some sensible data (Names, telephone numbers, addresses, personal photos etc.) in most of the folders

THE BIOSENSOR PLATFORM ITSELF!!!



iGEM teams and DIY-biologist contacted us to have the lab protocols and test the biosensor because they need one!

Most of the iGEM teams regardless of their project and their track selection need reliable data! they need to collect data fast and inexpensively, they need a biosensor!

The biosensor platform is our gift and the iGEM community! We spent hours and sleepless nights working on it and now its time to donate that to you! For the people to the people!


Stay safe and stay awesome!

Sincerely

Edinburgh's iGEm Team

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