Team:Edinburgh/Parts/Improvements

Team Edinburgh Finding NEMO

Improved Parts

The BBa_K190015 promoter was not very effective in transcription repression as evidenced by using it in the construct BBa_K3380603 . Therefore iGEM team Edinburgh 2020 decided to try and increase the transcription repression with ArsR transcription factor. We designed a promoter with the ArsR binding site placed after the promoter BBa_K3380103 . The newly designed promoter was tested in a Cupriavidus Metallidurans cell free extract expressing iSpinach ( BBa_K3380150 ) fluorescent RNA aptamer flanked by F30 tRNA scaffold ( BBa_K3380101 - BBa_K3380102 ).

			Name	Type	Description	Designer	Length
Our Part
		W	BBa_K3380103	Regulatory	T7 strong promoter with ArsR binding site	Alexandru Popov	70
Improved
		W	BBa_K190015	Regulatory	Arsenic Promoter (ArsR regulated)	Michael Verhoeven	70