Bronze Medal Criteria
- Our team has completed a project wiki, poster, presentation video, project promotion video, and judging form.
- Our team members were able to work in the wet laboratory (June 1st to July 31st; we were unable to access the lab prior to June 1st and August-October) in order to develop components of a detector system for the A2 genotype.
- Individual team member roles are listed on the Team Biographies page.
The contributions of faculty mentors, human practices partners, and team sponsors are provided on the Attributions page.
- Faculty mentors helped guide project design, taught experimental techniques, aided in troubleshooting, and helped with data interpretation.
- Human practice partners included farmers and those in the medical field whom team members interviewed in order to get information for our project.
- Our contributions are described on the Contributions page of our wiki.
- We have submitted the parts BBa_K3389000-BBa_K3389003 to the iGEM parts registry. We have characterized part BBa_K3389000. Limited access to laboratory resources prevented us from fully characterizing the other parts.
- We have produced an instructional manual with helpful tips for producing animated videos.
- We followed the engineering design cycle throughout our project. After researching ways of A2 gene detection, we imagined using the flappase enzyme combined with a light detector, designed and constructed a flappase detection system. We have made the most progress in optimizing the RPA assay that will be used to amplify the field samples that we aim to test. Work that remains to be done includes validating the function of our flappase enzyme and building a field-usable system for conducting the assay.
- Our team has collaborated with iGEM UPCH Peru and Moscow-Russia and have begun an iGEM cookbook project. These are described on our Collaborations page.
- The goal of our project is to make it easier for small farmers to enter niche markets in order to stay afloat. We consulted with local dairy farmers and medical professionals to design our project. We decided that synthetic biology could be used to make a field-usable detector that provides a cheaper and faster way to determine if a cow has the gene for A2 milk, a product with a growing market in the United States. The details of how these conversations informed our project design can be found on our Human Practices page.
- Our goal is for small farmers to be able to use our detector systems on their farms. We must design a system that is safe, simple to use, and temperature-stable. These considerations have been considered throughout our design. Plans for the field-usable product can be found on the Detection System page.
Integrated Human Practices
Improvement of an Existing Part
- To aid in the eventual optimization of our SNP detection system, we produced a model of the flappase enzyme from S. cerevisiae. This work is described in the Flappase section of our wiki.
Proof of Concept
- We developed the curricula and served as mentors for a virtual science camp for middle school students focused on Engineering with Biology, which is described on our Human Practices page.
Excellence in Another Area