With the advent of the era of biological information, obtaining accurate and reliable information has a significant effect on people’s life and production. Sensors are the main way and means to obtain nature and production. Among them, biosensors have already penetrated various sectors of the national economy. Food, pharmaceutical, chemical, medical and environmental monitoring etc. The biosensor has good specificity, easy operation, simple equipment, fast and accurate measurement, and wide application range.
A sensor is a detection device that can feel the information being measured, and can convert the sensed information into electrical signals, optical signals or other required forms of information output according to certain rules, to meet the display and Storage, recording and control requirements. The phosphorus sensor constructed by our project uses Escherichia coli as a carrier, which can sense the change of phosphate concentration in the environment, and thus display different light signals to indicate that the light signal is weak when the phosphate concentration is high, and when the phosphate concentration is low The light signal is strong, so the phosphorus concentration in the environment of E. coli can be easily and quickly detected.
Our phosphorus sensor can be used by water environment inspectors to detect the level of phosphorus concentration in the water environment; it can also be used by experimenters in the chemical industry to test the amount of phosphate added in the experimental system ; It can also be used in the pharmaceutical or fermentation field to detect phosphate consumption in the fermentation tank environment, etc. Our phosphorus biosensor has the following advantages:
(1) Using immobilized biological Escherichia coli as a carrier does not require expensive reagents, and can be used repeatedly, which overcomes the disadvantages of high cost of physical and chemical analysis reagents and complicated chemical analysis in the past;
(2) Strong specificity, only reacts to specific phosphate, and is not affected by color and turbidity;
(3)The analysis speed is fast, and the results can be obtained in a few minutes or ten minutes;
(4)High accuracy, generally the relative error can reach 1%;
(5)The operating system is relatively simple and easy to realize batch analysis;
(6) The cost is low. In continuous use, only a few cents of RMB is required for each case.
(7) In the production control or detection, many complex physical and chemical sensors can obtain information. At the same time they also pointed out the direction to increase the yield of the product.
Of course, there are still many areas for improvement in our phosphorus receptors, such as the activity of E. coli, and how to ensure the normal growth of E. coli without affecting the life activities of other microorganisms in the relevant growth environment, that is, how to protect the large intestine The safety of bacillus phosphorus receptors, these problems still need us to think and solve. Besides, we also face many unknown challenges, such as how to control the growth and reproduction of E. coli, how to make our phosphorus receptors more intelligent and diversified, and how to reduce the cost of cultivating E. coli and producing phosphorus receptors. How to improve the detection sensitivity of this sensor? I believe that the improvement of these problems will accelerate the process of marketization and commercialization of our phosphorus sensor.
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