Team:Shanghai HS/Notebook
Notebook
Day1
Online training to introduce the background of the subject and related knowledge.
Day2
（1）The coach leader introduces himself and introduces the training content；
（2）ice breaker games；
（3）Candidates for captain election, determine the team name, logo, slogan, uniform and related surroundings；
Day3
Laboratory safety and basic operation training, molecular biology, protein and cell biology and other biological knowledge training;
Understand the common instruments and equipment in biochemical laboratories, such as centrifuges, PCR machines, sterilization pots, constant temperature shakers, etc., and practice the use of common instruments, such as micropipettes, etc.
Day4
Understand the chemical reagents and drugs needed for experiments, learn the use of balances, measuring cups, and magnetic stirrers, and practice the preparation and sterilization of culture media
Day5
PCR, and agarose gel Recycle
Learn the preparation of the PCR reaction system and the use of the PCR machine, learn the role and function of the relevant reagents in the PCR reaction, understand the specific steps of the PCR reaction program, and be able to operate independently.
Day6
Enzyme digestion, connection, transformation
Learn how to use DNA restriction endonucleases and the principle of action, understand the knowledge of DNA connection and the principle of E. coli transformation, and understand the basic concepts and operations of genetic engineering
Day7
Pick bacteria, PCR identification
Learn the relevant knowledge and operation of colony PCR, understand the methods and principles of PCR identification, and learn the principles and related operations of agarose gel electrophoresis
Day8
Sample delivery for sequencing
Perform sequencing analysis on the correct bacteria identified by PCR, verify the correctness of the DNA sequence, and understand the relevant knowledge and principles of gene sequencing
Day9-10
Escherichia coli plasmid extraction
Learn the relevant knowledge of E. coli plasmid extraction, understand the reagent components in the plasmid extraction kit and their related functions, and be able to operate proficiently
Day11
(Analysis of sequencing results), phosphate treatment
According to the results of second-generation sequencing, select the vector to construct the correct Escherichia coli and treat it with different concentrations of phosphate; then select the appropriate phosphate concentration and process for different times to obtain the response conditions of the PhoA gene
Day12
Fluorescence detection, microplate reader detection
Perform fluorescence detection and analysis on the obtained E. coli samples, and learn how to use the microplate reader
Day13
Data processing
Perform relevant processing of experimental data, learn how to use related software, analyze experimental results, make preliminary judgments on experimental results, analyze the pros and cons of experimental design, find problems in the experimental process and propose optimization measures
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