CMV-Replaceable-1 -Fluc-P2A-Rluc(Part: BBa_K3396016)
Usage and Biology
(Part:BBa_K3396008)is a Dual Luciferase Reporter system to quantify the abundance of specific target protein. Dual luciferase assay was introduced to normalize the differences caused by irrelevant factors.
Special designs were taken to optimize the applicability and adaptivity of such parts. Specifically, a novel designed substitution system, through which, target proteins could be fused with Fluc fragment using Golden-Gate Assembly, was introduced to dramatically simplify the cloning process.
We use Golden-Gate Assembly to fuse GFP with Fluc fragment. This system can be used to quantify the abundance of specific target protein. We compared the degradation curves of GFP and Fluc as reporter molecules, respectively and result showed that the detection of Fluc is more accurate.
Figure 2.Detection accuracy of different reporter system.