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| <p style=" text-align: justify;font-size:16px"><strong style="font-size:16px">Figure 2.GFP Predator Pro system based on the DocS-Coh2 interaction module. </strong> (A) Schematic representation showing the modularized design and function of GFP Predator Pro (GFP PrePro.) (B) Schematic representation showing the flow chart of dosage optimization and its results. The fluorescent intensity of GFP in each well was shown with heatmap. (C) Fluorescence images and intensity quantification of the GFP-Predator Pro transfected group and its negative control transfected with an empty vector. HEK293T cells in both groups were transfected with GFP-expression plasmid. (D) Western blotting determining the expression level of GFP in HEK293T cells transfected with GFP Predator Pro. (E) Model of degradation effect over time. (F) Fluorescence images showing the degradation of GFP in GFP Predator transfected groups at different times after transfection. N, P represents Negative control and GFP Predator, respectively. </p> | | <p style=" text-align: justify;font-size:16px"><strong style="font-size:16px">Figure 2.GFP Predator Pro system based on the DocS-Coh2 interaction module. </strong> (A) Schematic representation showing the modularized design and function of GFP Predator Pro (GFP PrePro.) (B) Schematic representation showing the flow chart of dosage optimization and its results. The fluorescent intensity of GFP in each well was shown with heatmap. (C) Fluorescence images and intensity quantification of the GFP-Predator Pro transfected group and its negative control transfected with an empty vector. HEK293T cells in both groups were transfected with GFP-expression plasmid. (D) Western blotting determining the expression level of GFP in HEK293T cells transfected with GFP Predator Pro. (E) Model of degradation effect over time. (F) Fluorescence images showing the degradation of GFP in GFP Predator transfected groups at different times after transfection. N, P represents Negative control and GFP Predator, respectively. </p> |