Team:Leiden/Attributions
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Rapidemic
Attributions
The iGEM 2020 team would like to acknowledge the contributions of all of those who helped us in our project with fundraising, laboratory support and other general support. Without their help our we would not have been able to advance so far in our project. Nonetheless, they also helped making our experience of the project incredibly fun! Thanks to all people and organisations for their contributions.
Principal Investigators
- Prof. dr. Han de Winde
- Dr. Dennis Claessen
Supervisors
- Laurens ter Haar
- Marjolein Crooijmans
- Floor Stel
- Maarten Lubbers
- Jonah Anderson
Advisors
- Chiel van Amstel
- Daniël Tan
- Tobias Fecker
- Jo-Anne Verschoor
- Daan van Tol
- Charlotte de Ceuninck
- Carli Koster
Science
- Erik Vijgenboom for his advice on substitutes for DMSO and improving the false positive ratio.
- Paul van Heusden for providing Saccharomyces cerevisiae genomic DNA.
- The Claessen lab for their constructive and useful feedback on our science presentations and communications.
- Germaine Aalderink for help with the preliminary and final safety form
Lab Practicalities
- Ellen de Waal and Ilja Dekker from the IBL for helping us to order the supplies we required for our experiments.
- Mark Arendtshorst from the IBL for the use of and his instruction on how to operate the plate reader.
- Kees Koops from the IBL for allowing us to work in his laboratory for our experiments over the summer.
- Hein van der Meer from the IBL for his positive attitude and warm welcome every morning at the Sylvius Laboratory during our summer lab work.
Outreach
- Annette Heijn for helping us set up crowdfunding and reaching Leiden University's alumni network.
- Annelien Zweemer, Eveline Hoebe, Pieter Hiemstra, Jeroen van Smeden, Coen van Hasselt, Kees van den Berg, Eric Snijder, Mionne Laman, Merel Rem for sharing our crowdfunding with their community.
- For our general outreach, we would like to thank all news outlets and other organizations involved: Leiden University, Leidsch Dagblad, Sleutelstad FM, BNR Radio, Medical Delta, CDL, S.V. LIFE, Bionieuws, Voorschotense Krant, Youth Educational Forum, K.N.P.S.V. and NEMO.
Human Practices
- Dr. Peter Bredenbeek for sharing his expertise with us on PCR, LAMP and the characteristics of the point-of-care device.
- Edouard Bonneville for his help finetuning our model.
- Niek Savelkoul for sharing his experiences and the lessons he learned while he was member of Mantis 2017.
- Hélène van Oorschot for her knowledge about and experience with infectious diseases she gained during her internship in Ghana.
- Heleen Koudijs for her knowledge about and experience with infectious diseases she gained as a tropical doctor in Africa (Tanzania, Zambia, Ethiopia).
- John Tonkinson for his insights on kit design, the ideal user of our kit and how best to enter the African market.
- Dr. Dam for her insights as a general practitioner regarding kit design, user-friendliness, and the end-users of our kit.
- Ward Heij for help to understand the international point-of-care rapid diagnostic kit market.
- Sophie van Baalen for her insights on ethics, societal consequences as well as the end-user of our kit.
- Aldrik Velders for his help with the application process as well as kit design.
- Dr Assica Permata for her insights working as a physisian in Indonesia on the need for a point-of-care rapid diagnostic kit as well as her insights on kit disposal in Indonesia.
- Armand Paauw & Hans van Leeuwen for their input on kit disposal, for helping us choose an application, and their tips for kit design.
- Dr Otto Kroesen for help to understand the African market not only econimcically but also with regards to societal consequences, cultural differences and logistical issues
- Red bag solutions for showing us how medical waste could be disposed of in the future.
- René Paulussen for sharing his expertise on ways how to enter the market, the logistical issues in Africa as well as his views on certain applications.
- Bram de Graaf for showing us how (medical) waste is transported in the Netherlands as well as his views on sustainability in kit design.
- Pim Martens for his help regarding choosing an application.
- Huram Konjen for bringing to light where diagnostics need improvement.
- Eric de Coster for giving us insight into how the information infrastructure and data collection work regarding diseases in the Netherlands.
- Willem Mees van der Bijl for his advice in the application process as well as notifying us about the importance of how geography plays a role in a fierce epidemic but not yet pandemic situation.
- Robin van Houdt for his insight on how rapid diagnostic test kits can be useful in hospitals and sophisticated hospital laboratories in developed countries.
- Ron Roffel for giving us a tour of the sole medical waste disposal company of the Netherlands and therefore showing us how medical waste is disposed of.
- Eric van Vught for his insights how lyophilizing reagents affect cost as well as kit design
- Janneke van de Wijgert for her insights on the low availability of rapid diagnostic test kits in low-resource countries as well as her insights on the reliability of various test methods.
- Guus Eskens for his insights on the implementation of our kit in developing countries.
- Corona patients, malaria patients, dengue fever patients, and the TB patients for their insights on their thoughts and behavior change when diagnosed positively, their experience during diagnosis, their thoughts on themselves as end-users as well as the difficulties they faced in the diagnostic procedure.
Collaborations
Teams that we have worked with during our collaborations. Team FCB-UANL, team Aalto-Helsinki, team Rochester, team MSP, team RUM and team UPCH for presenting at our iGEM symposia. Furthermore, team MSP, team Groningen, team Lausanne UNIL, team Chalmers Götenborg, team Uppsala, team Darmstadt, team Aachen, team Delft, team Warwick and team TAS_Tapei for setting up collaborations that we have participated in.
Entrepreneurship
We want to thank Anastasiia Tupitcyna, Andrew Wolf, and Zuza Nazaruk for reviewing the entrepreneurship page. We would also like to thank Willem Mees van der Bijl and Alaitz Izaguirre from IDE Netherlands for their mentorship on RDT kit design and commercialization during the project.
Our contribution
Experimental work
| Name | Project Part | Task description |
|---|---|---|
| Tom Langelaar | GQ-oxidation, RPA and LAMP | Proof of concept study for false-positives. GQ activity-concentration relationship. Primer validation using real time RPA. Initial characterization LAMP assay. |
| Sebastian Tandar | RPA, LSDA, GQ-Oxidation, Proof-of-Concept | Initial characterization GQ oxidation and LSDA. Co-developed proof of concept. Investigated causes of false-positives and effects of DMSO on RPA selectivity. |
| Marijn van den Brink | RPA, LSDA, GQ-Oxidation, Proof-of-Concept | Initial characterization GQ oxidation and LSDA. Co-developed proof of concept. |
| Sinisha Jovic | RPA | Initial characterization, primer validation, kinetics study for RPA. |
| Tim van den Akker | RPA, Proof-of-Concept | Initial characterization, primer validation, sensitivity and kinetics study for RPA. Assisted in Proof-of-Concept validation. |
| Kelly van Strien | RPA | Sensitivity and selectivity studies for RPA. Primer validation using real-time RPA. |
| Amber Schonk | RPA | Sensitivity and selectivity studies for RPA. Primer validation using real-time RPA. |
| Tijn Delzenne | Proof-of-Concept | Proof of concept study for false-positives. |
| Violette Defourt | RPA, Proof-of-Concept, GQ-Oxidation | Co-developed proof of concept. Sensitivity for RPA. Paper-based GQ Oxidation. |
| Marjolein Crooijmans | Technical Support | Thanks for all your help in the lab! |
Fundraising and Finances
Our finance managers Lucy Chong and Tom Langelaar were in charge of our fundraising, finance, and managed our budget. Tom Langelaar called various companies. Lucy Chong managed the crowdfunding. Therefore, she did interviews with newspapers and wrote articles for study associations.
Design
Our Design Manager, Tijn Delzenne, was in charge of the making of visuals for the project. With help from numerous team members an overall style for the project was created that can be seen throughout the whole project. Tijn learned Illustrator and After Effects and, with help from others, created the logo, made animations for the videos, designed info graphics and much more. Much of his work can be seen throughout this website.
Collaborations
Our Design Manager, Tijn Delzenne, was in charge of the making of visuals for the project. With help from numerous team members an overall style for the project was created that can be seen throughout the whole project. Tijn learned Illustrator and After Effects and, with help from others, created the logo, made animations for the videos, designed info graphics and much more. Much of his work can be seen throughout this website.
Programming and Statistics
The wiki programming was done by Kelly van Strien. Data analysis and mathematical modeling in R was performed by Sebastian Tandar and Marijn van den Brink.
Outreach and Social Media
The Communication Manager, Amber Schonk, and Public Engagement Manager, Violette Defourt, have worked closely together to maintain the social media platforms of the project. Amber was in charge of the newsletter and outreach to various media, such as manage contacts with local newspapers and writing blogs. Violette was mainly involved with the educative aspects of the project, such as making videos, providing content for social media and writing articles.
Lab & Safety
Tim van den Akker was responsible for the safety in the laboratory, filling out our safety form and for ordering all the experimental supplies.
Presentations
Presentations for the wide public and smaller scientific groups were given by Amber Schonk, Violette Defourt, Sebastian Tandar, Tim van den Akker and Marijn van den Brink.
Wiki
The wiki was programmed and designed by Kelly van Strien. All team members contributed to writing the content, however, the final content was managed by Aukje Beers.
Wiki Pages
| Page name | Written and Designed by |
|---|---|
| Attributions | Lucy Chong, Joey Meijdam |
| Collaborations | Amber Schonk |
| Contribution | Aukje Beers, Marijn van den Brink, Eugene Golov |
| Description | Tim van den Akker, Marijn van den Brink, Lucy Chong |
| Engineering | Marijn van den Brink, Sebastian Tandar |
| Entrepreneurship | Marijn van den Brink, Violette Defourt, Eugene Golov, Tom Langelaar, Kelly van Strien |
| Experiments | Tim van den Akker, Marijn van den Brink, Kelly van Strien |
| Featured | Amber Schonk, Kelly van Strien |
| Hardware | Tim van den Akker, Aukje Beers, Marijn van den Brink, Eugene Golov, Sebastian Tandar |
| Human practices | Aukje Beers, Violette Defourt, Joey Meijdam, Kelly van Strien |
| Implementation | Aukje Beers, Lucy Chong, Violette Defourt |
| Inclusion | Aukje Beers, Tijn Delzenne, Kelly van Strien |
| Main page | Tim van den Akker, Aukje Beers, Lucy Chong, Tijn Delzenne, Amber Schonk, Kelly van Strien |
| Medal Criteria | Kelly van Strien |
| Models | Marijn van den Brink, Kelly van Strien, Sebastian Tandar |
| Newsletter | Amber Schonk |
| Notebook | Tim van den Akker, Marijn van den Brink, Tijn Delzenne, Sinisha Jovikj, Tom Langelaar, Amber Schonk, Kelly van Strien, Sebastian Tandar |
| Parts | Marijn van den Brink |
| Proof of concept | Marijn van den Brink, Sebastian Tandar |
| Public engagement | Violette Defourt |
| Registry | Marijn van den Brink |
| Results | Tim van den Akker, Marijn van den Brink, Sinisha Jovikj, Sebastian Tandar, Kelly van Strien |
| Safety | Tim van den Akker |
| Sponsors | Aukje Beers, Lucy Chong, Tom Langelaar, Kelly van Strien |
| Team members | All |