Team:NTHU Taiwan/Contribution
Overview
1. The feasibility of method to detect quantum dot by fluorescence spectrum.
2. The Improvement of E.coli tolerance ability(Cadmium ion).
3. Verify yhaO can help produce QD.
4. Simulate intracellular nucleation through PFC model
5. Open wiki source for future iGEM team
Contribution
1. Check the feasibility of method to detect quantum dot by fluorescence spectrum
There are two traditional methods to detect quantum dots: Fluorescence spectrum and Electron microscope(SEM/TEM). But these ways were only allowed in the sample from chemical synthesis. Our team find that it will be successful using fluorescence spectrum way by using M9 Minimal Medium. We also find the effective area of fluorescence detection, which is 450nm and 500-600nm area(Fig1-1、Fig1-2).
2. Check the Cadmium ion tolerance ability of MT2 peptide
In order to use E.coli to help us make a heavy metal production, we need to improve the tolerance ability of metal ions. Our team transfers a kind of peptide gene-MT2 from colocasia esculenta(a kind of hydrophytes). We find that this peptide will be successful synthesized after 3 hours when we added Cd ion into solution. MT2 can help E.coli tolerates ≤10^-5M Cd ion(concentration).
3. Verify yhaO can help produce QD.
Quantum dots are metal sulfide particles, so it is necessary to provide a source of sulfur ions. We transferred the yhaO protein gene into JM109. This enzyme converts cysteine into hydrogen sulfide. With the help of this enzyme, bacteria can produce quantum dots more efficiently(Fig3).
4. Simulate intracellular nucleation through PFC model
We introduce the PFC model to simulate intracellular nucleation and this method can be extend to other crystal growth in cell. The benefit of setting simulation is the simple parameter of this model, which let us simulate the clearly acquainted of the bio-system. The further iGem team can use this model to do all similar intracellular nucleation simulation even when they know little about the mechanisms.
5. Open wiki source for future iGEM team
Our team's wiki code is open for everyone to view source through the iGEM topbar. Each section is well-organized in the view source. The further iGEM team can use the HTML code and CSS code for their demand.
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