Team:NTHU Taiwan/Engineering
Successfully Gene Cloning
In order to initially verify our cloning result. We use chloramphenicol as our C antibiotics to test the plasmid functionality. By the method, we are able to diminish the effect by other types of bacteria or contamination. After growing in the incubator for 24 hours. A significant amount of colony indicate the success of cloning.
Cadmium Ion Transportation Ability of MntH protein
In design part, we attach promoter cada with red fluorescence protein to detect the curve of fluorescence intensity (excited by wavelength 590 nanometer, emission of wavelength 630 nanometer versus time), the figure shows us the cada combine with RFP shows much higher FLU value when compare to JM109 control group. Furthermore, when cada also ligase with RFP and MntH, the increase amount of cadmium ions trigger the promoter to produce even higher amount of RFP. Result in the greater FLU value.
We introduce gene MntH as the channel protein for cadmium ions as well as balance the concentration between inner and outer membrane. The figure below shows the growth curve of E.coli contain MntH is getting lower with increase of cadmium concentration. Indicating the MntH gene is function with transport more cadmium ions into the cell.
Cadmium Ion Tolerance Ability of MT2 peptide
MT2 are proved to lower the toxicity cause by cadmium ions. With detail figure below, we can observe O.D.600 value is relatively higher than those without MT2.
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