BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All parts were generated during our project.
Table 1. Tools used in the dry lab part of our bioreactor. All electrical equipment used was designed to fit for assembly with Biosquad.
|BBa_K1724000||CadA promoter (Cd2+)||Promoter||31 bp|
|BBa_K525998||Promoter T7 and RBS||Promoter||32 bp|
|BBa_R0010||promoter (lacI regulated)||Promoter||55 bp|
|BBa_K1526007||MntH transporter from NRAMP family||Coding||2676 bp|
|BBa_K2242233||Cysteine Desulfhydrase (yhaO)||Coding||1203 bp|
|BBa_E1010||Red Fluorescent Protein||Coding||706 bp|