We have contributed 26 new parts to the registry. All of our parts are included in our Yarrowia lipolytica collection, which includes various basic parts for general genetic circuit construction, devices containing cellulase proteins that have been optimized for high production and secretion, reporter constructs, and other useful devices. Our devices can be connected to each other in a variety of ways using Gibson Assembly (learn more about Gibson Assembly by scrolling down this page).
We have also added literature-reviewed characterization to the promoter BBa_K2117000, and have planned our own experiments to add further data to this part once we have further lab access. The characterize page also includes in-depth information on our two featured parts: BBa_K3629013 and BBa_K3629016. These two parts are chimeric proteins that have been modelled and enhanced for function in Y. lipolytica.
Gibson Assembly is a molecular cloning technique that allows multiple DNA fragments with overlapping ends to be joined together in a single isothermal reaction. Gibson utilizes a 5’ exonucleases to digest the 5’ end of the DNA fragments and create homologous overhangs. DNA fragments then come together in the correct order through complementary base pairing of their overhangs. In the final step of Gibson Assembly, DNA polymerase fills in any remaining gaps, and ligase glues the DNA fragments together. Gibson Assembly has become an extremely useful DNA assembly technique due to its ability to efficiently assemble very large and complex genetic constructs in one simple reaction.
Figure 1. Diagram of how Gibson Assembly works.
Below is the list of parts we submitted to the registry this year.