Antibiotic resistances are one of the main global health problems worldwide. These resistances
come from their massive use as a preventive and therapeutic treatment.
Pseudomonas aeruginosa accounts for one of the most problematic/threatening bacteria partly due to its ability to form a 3D protective extracellular matrix: a BIOFILM
P. aeruginosa causes many health problems essentially because of its biofilm such as nosocomial, wound or burn infections, otitis, etc.
Introducing PyoBusters biotreatment
Our engineered E. coli can sense P. aeruginosa’s biofilm employing its Quorum Sensing. In presence of this biofilm, E. coli's population grows which constitutively produces and accumulates therapeutics molecules stocked inside them. At a threshold, the bacteria will be lysed and will deliver the therapeutics molecules on the biofilm. These steps can be repeated until the total destruction of P. aeruginosa.
How does it work?
The delivery system is an essential part of PyoBusters. This system is based on E. coli’s own quorum sensing molecule: AI-2. When it reaches a threshold population it will trigger the production of the lysis protein and allow therapeutic molecules to be released through the lysis of PyoBusters.