Team:Grenoble Alpes/Parts

PyoBuster - Parts

Parts

This year our team needed to work with several parts listed below. The choice was relatively limited as we only had the plates from previous years available because of the pandemic. We therefore designed new parts to face our problems including RBS, two new coding sequences: Alginate lyase and Pyocine S5 and a new backbone.

iGEM parts used already existing:

Name Type Description Designer Length
BBa_1732100 Protein coding sequence LacI Grace Kenney 1153 bp
BBa_K1949060 Promoter PrhlR Yoshio Takata 799 bp
BBa_C0171 Protein coding sequence RhlR jcbraff 729 bp
BBa_K649100 Promoter Plsr Hiroki Yoshise 97 bp
BBa_2572025 Promoter Ptac Shan Jiang 56 bp
BBa_J23100 Promoter J23100 john Anderson 35 bp
BBa_J23106 Promoter J23106 john Anderson 35 bp
BBa_J23102 Promoter J23102 john Anderson 35 bp
BBa_B0015 Terminator B0015 Reshma Shetty 129 bp
BBa_B0017 Terminator B0017 Austin Che 168 bp
BBa_K1094400 Protein coding sequence eGFP Emil Christian Fischer 723 bp
BBa_K1659200 Protein coding sequence Dispersin B Wei Chung Kong 1110 bp
BBa_J06504 Protein coding sequence mCherry ytwang 714 bp

New iGEM parts used:

Name Type Description Designer Length
BBa_K3463001 Coding Pyocin S5 Noémie Garnier 1497 bp
BBa_K3463002 Coding algL-Alginate Lyase Noémie Garnier 1310 bp
BBa_K3463007 Composite J23100 RBS RhlR Mathieu Clavel 68 bp
BBa_K3463008 Composite J23100 RBS RhlR B0015 Mathieu Clavel 893 bp
BBa_K3463011 Composite PrhlR RBS eGFP Mathieu Clavel 1547 bp
BBa_K3463017 Composite Ptac RBS low GFP B0017 Elouen Le Garrec 977 bp
BBa_K3463018 Composite Ptac RBS high GFP B0017 Elouen Le Garrec 977 bp
BBa_K3463019 Composite J23100 RBS RhlR B0015 PrhlR RBS eGFP Mathieu Clavel 3987 bp
BBa_K3463020 Composite Ptac RBS GFP B0017 J23100 RBS RhlR B0015 PrhlR RBS LacI Elouen Le Garrec 3094 bp
BBa_K3463023 Composite Plsr RBS high mCherry Sara Salhi 851 bp
BBa_K3463024 Composite Plsr RBS low mCherry Sara Salhi 837 bp
BBa_K3463025 Composite Plsr + RBS 100+ E7 ( 2 007 898) Sara Salhi 280 bp
BBa_K3463026 Composite Plsr + RBS 50 + E7 (997 965) Sara Salhi 266 bp
BBa_K3463027 Composite Plsr + RBS 25 + E7 ( 456 093) Sara Salhi 266 bp
BBa_K3463028 Composite Ptac RBS 100% E7 Elouen Le Garrec 230 bp
BBa_K3463029 Composite Ptac RBS 50% E7 Elouen Le Garrec 230 bp
BBa_K3463030 Composite Ptac RBS 25% E7 Elouen Le Garrec 230 bp
BBa_K3463031 Composite PrhlR RBS LacI Elouen Le Garrec 1910 bp
BBa_K3463000 Plasmid_Backbone pUCBB-eGFP Iryna Moskalevska 3174 bp
BBa_K3463003 RBS RBS eGFP and RhlR (RhlR construction) Mathieu Clavel 25 bp
BBa_K3463004 RBS J23102 RBS eGFP Mathieu Clavel 758 bp
BBa_K3463005 RBS J23106 RBS eGFP Mathieu Clavel
BBa_K3463006 RBS J23100 RBS eGFP Mathieu Clavel
BBa_K3463009 RBS RBS eGFP Mathieu Clavel 25 bp
BBa_K3463010 RBS RBS LacI Elouen Le Garrec 25 bp
BBa_K3463012 RBS Ptac RBS GFP low Elouen Le Garrec 25 bp
BBa_K3463013 RBS Ptac RBS GFP high Elouen Le Garrec 25 bp
BBa_K3463014 RBS Ptac RBS GFP low Elouen Le Garrec 25 bp
BBa_K3463015 RBS Ptac RBS E7 50% Elouen Le Garrec 25 bp
BBa_K3463016 RBS Ptac RBS E7 25% Elouen Le Garrec 25 bp
BBa_K3463021 RBS RBS mCherry High Sara Salhi 26 bp
BBa_K3463022 RBS RBS mCherry low Sara Salhi 26 bp