Difference between revisions of "Team:Calgary/Results"
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<a href="#parts">Parts</a> | <a href="#parts">Parts</a> | ||
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| − | <a href="#modelling">Modelling</a> | + | <a href="#modelling">Modelling, Software, and Measurement</a> |
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<a href="#future-directions">Next Year</a> | <a href="#future-directions">Next Year</a> | ||
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<div class = "modelling" id="modelling"> | <div class = "modelling" id="modelling"> | ||
| − | <h2>MODELLING</h2> | + | <h2>MODELLING, SOFTWARE, AND MEASUREMENT</h2> |
<h4>Bellatrix</h4> | <h4>Bellatrix</h4> | ||
<a href="Bellatrix"><p style="font-size: 80%; font-weight: bold; color:#0197AE;">Go to Bellatrix page </p></a> | <a href="Bellatrix"><p style="font-size: 80%; font-weight: bold; color:#0197AE;">Go to Bellatrix page </p></a> | ||
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<a href="GausHaus"><p style="font-size: 80%; font-weight: bold; color:#0197AE;">Go to GausHaus page </p></a> | <a href="GausHaus"><p style="font-size: 80%; font-weight: bold; color:#0197AE;">Go to GausHaus page </p></a> | ||
<p> | <p> | ||
| − | + | GausHaus was not like the other software sitting by the beach enjoying sparkling cider; it put in the work. GausHaus spent the summer confusing and terrorizing our statistician with sprinkles of success amongst an ocean of setbacks. Once it clicked though, we were off to the races with GausHaus coming through whenever called upon. This resulted in it being instrumental to our protein modelling, allowing the quantification of SEGI-8s dynamics along all three axes. It then also served as a software contribution to the iGEM community. And just when we thought it was done, it hit us. GausHaus' quantification of protein dynamics is an ideal measurement for teams during this renaissance of computational biology brought on by COVID-19. All in all we fought, we loved, and we demolished some molecular dynamics. What more could you ask for? | |
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| − | + | ||
| − | + | ||
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</p> | </p> | ||
<h4>Metabolic Flux</h4> | <h4>Metabolic Flux</h4> | ||
Revision as of 22:21, 27 October 2020
Overview
Engineering Success
Human Practices
Proposed Implementation
Parts
Modelling, Software, and Measurement
Next Year
OVERVIEW
The following page describes the summarized results of our work in each of our subprojects. Please visit the corresponding links to the subproject specific pages to get more details on the background information and our methods.
ENGINEERING SUCCESS
Cellulase Integration
Go to Cellulase page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Biocontainment
Go to Biocontainment page
We developed a sustainable and affordable biosafety system where two yeast strains will be co-cultured together in a syntrophic community. These strains will each be auxotrophic for a molecule the other strain has been modified to overproduce. This ensures that the two strains are co-dependent, reducing the chances of environmental escape. The organism can only proliferate if both strains are present in sufficient quantities to support each other’s growth. Ultimately, this novel method of co-dependent auxotrophy provides an easily implementable biocontainment strategy to prevent the escape of genetically engineered organisms.
This season specifically, we developed a proof of concept for our co-culturing system using a pair of already engineered S. cerevisiae strains. We are happy to say that by the end of this season, we accomplished quite a bit:
✔ We successfully characterised a proof of concept for our biocontainment system using a pair of already engineered S. cerevisiae strains:
- Characterised the strains in terms of auxotrophy and amino acid overproduction
- Created a viable co-culture demonstrating the ability of the strains to survive using the amino acid released by each of the complementary strains
- Demonstrated the inability of the yeast to survive in environmental conditions outside of the expected growth vessel conditions
✔ We designed and submitted a total of eight parts to the parts registry that allow for the engineering of Y. lipolytica to create complementary overproducing, auxotrophic strains.
Thymol Production
Go to Thymol page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
HUMAN PRACTICES
Creating a human-centred project
Go to Human Practices page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
PROPOSED IMPLEMENTATION
Bioreactor
Go to Bioreactor page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Randle's Cell Testing Device
Go to Randle Cell Testing Device page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Entrepreneurship
Go to Entrepreneurship page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
ENGAGEMENT
Education
Go to Education page
We successfully developed and executed an introductory synthetic biology course at the undergraduate level--the first synthetic biology-specific course offered at our institution. This has helped bring more attention to the field of synthetic biology to faculty members and students alike. Our course included a variety of assignments, laboratory experiments, and group work to train new iGEM members for the season. Furthermore, we dedicated ourselves to helping iGEM teams by providing mentorship on human practices and team organization. We also developed a branding style guide that we implemented in our own project this season, which promotes scientific communication to general audiences. You can learn more here.
Collaborations
Go to Collaboration page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
PARTS
Characterize
Go to Parts Characterization page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
MODELLING, SOFTWARE, AND MEASUREMENT
Bellatrix
Go to Bellatrix page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Protein Modelling
Go to Modelling page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
GausHaus
Go to GausHaus page
GausHaus was not like the other software sitting by the beach enjoying sparkling cider; it put in the work. GausHaus spent the summer confusing and terrorizing our statistician with sprinkles of success amongst an ocean of setbacks. Once it clicked though, we were off to the races with GausHaus coming through whenever called upon. This resulted in it being instrumental to our protein modelling, allowing the quantification of SEGI-8s dynamics along all three axes. It then also served as a software contribution to the iGEM community. And just when we thought it was done, it hit us. GausHaus' quantification of protein dynamics is an ideal measurement for teams during this renaissance of computational biology brought on by COVID-19. All in all we fought, we loved, and we demolished some molecular dynamics. What more could you ask for?
Metabolic Flux
Go to Metabolic Flux page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Measurement
Go to Measurement page
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
NEXT YEAR
Our goals for next year
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
