Difference between revisions of "Team:Calgary/Experiments"
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| − | <li> | + | <li>Mix 100ml of 1X TAE buffer with 1g of regular or LMP agarose (for a 1% gel). For a small gel, mix 30ml of 1X TAE with 0.3g agarose.</li> |
| − | <li> | + | <li>Microwave covered for about 1.5 minutes or until agarose is dissolved and pour into a balanced casting tray. LMP gels should be cast in the fridge. </li> |
| − | + | <li>Add a volume of Safe-Red dye to be 1/6th the total volume of the sample. </li> | |
| − | + | <li>Run samples at 100V (regular gel) or 80V (LMP gel) until sample is ¾ of the way down the gel. </li> | |
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| − | + | ||
| − | + | ||
| − | <li> | + | |
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Revision as of 07:22, 3 October 2020
General Wet Lab: E. coli Protocols
TEF1 Promoter Testing
Cellulase Secretion
Omega-3 Production
Biosafety
General Wet Lab: E. coli Protocols
- Mix 100ml of 1X TAE buffer with 1g of regular or LMP agarose (for a 1% gel). For a small gel, mix 30ml of 1X TAE with 0.3g agarose.
- Microwave covered for about 1.5 minutes or until agarose is dissolved and pour into a balanced casting tray. LMP gels should be cast in the fridge.
- Add a volume of Safe-Red dye to be 1/6th the total volume of the sample.
- Run samples at 100V (regular gel) or 80V (LMP gel) until sample is ¾ of the way down the gel.
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TEF1 Promoter Testing
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Cellulase Secretion
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Omega-3 Production
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Biocontainment
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