Difference between revisions of "Team:Calgary/Cellobiohydrolase"

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Cellobiohydrolase(CBH) is the second cellulase in our cellulose degradation efforts. After the endoglucanase has finished with the cellulose, CBH cleaves units off of the end. This is accomplished by breaking the celluloses 1,4-beta-D-glycosidic bonds. This then leaves the substrate primed for beta-glucosidase.
 
Cellobiohydrolase(CBH) is the second cellulase in our cellulose degradation efforts. After the endoglucanase has finished with the cellulose, CBH cleaves units off of the end. This is accomplished by breaking the celluloses 1,4-beta-D-glycosidic bonds. This then leaves the substrate primed for beta-glucosidase.
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CBHs are commonly comprised of three different components. The first is a catalytic domain responsible for the enzymatic activity of the cellulase. Next, there is the cellulose-binding module that anchors the cellulase to the substrate. The final component is a flexible linker region that connects the two. All three of these components contribute to the efficiency, and therefore the components and their interactions with each other will be modelled for better understanding.
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<img class="img-fluid" src="https://static.igem.org/mediawiki/2020/2/25/T--Calgary--Cellulo.png">
 
<img class="img-fluid" src="https://static.igem.org/mediawiki/2020/2/25/T--Calgary--Cellulo.png">
 
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<p>
 
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          In order to provide a sustainable, community-based solution, we plan
 
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          to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous
 
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          yeast that naturally produces beta-carotene and lipids, to be more
 
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          robust and resource-efficient. By modifying the yeast to produce
 
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          cellulase, it can then use common agricultural waste products as an
 
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          energy source for synthesizing its oil. It can then be eaten as a
 
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          vitamin A supplement. The yeast strain, while naturally safe and
 
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          non-pathogenic, will also be genetically modified to include a kill
 
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          switch for bio-containment, and optimized for oil production.
 
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        </p>
 
  
  

Revision as of 07:05, 18 October 2020



WHAT IS CELLOBIOHYDROLASE

What impact does it have in our project

Cellobiohydrolase(CBH) is the second cellulase in our cellulose degradation efforts. After the endoglucanase has finished with the cellulose, CBH cleaves units off of the end. This is accomplished by breaking the celluloses 1,4-beta-D-glycosidic bonds. This then leaves the substrate primed for beta-glucosidase.

CBHs are commonly comprised of three different components. The first is a catalytic domain responsible for the enzymatic activity of the cellulase. Next, there is the cellulose-binding module that anchors the cellulase to the substrate. The final component is a flexible linker region that connects the two. All three of these components contribute to the efficiency, and therefore the components and their interactions with each other will be modelled for better understanding.



PRIMARY STRUCTURE

Getting the Sequence Right

In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.


STRUCTURAL PREDICTION AND INTEROGATION

What are the loops doing?

In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

RESULTS

What we accomplished

In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.