Difference between revisions of "Team:Calgary/Parts"

Line 209: Line 209:
 
         <br>
 
         <br>
 
         <p>
 
         <p>
−
           We have contributed 26 new parts to the registry and added new characterization to 1 existing part. All of our parts are included in our <span style="font-style: italic;class="italic">Yarrowia lipolytica</span> collection, which includes various basic parts for general genetic circuit construction, devices containing cellulase proteins that have been optimized for high production and secretion, reporter constructs, and other useful devices. Our devices can be connected to each other in a variety of ways using Gibson Assembly.
+
           We have contributed 26 new parts to the registry. All of our parts are included in our <span style="font-style: italic;class="italic">Yarrowia lipolytica</span> collection, which includes various basic parts for general genetic circuit construction, devices containing cellulase proteins that have been optimized for high production and secretion, reporter constructs, and other useful devices. Our devices can be connected to each other in a variety of ways using Gibson Assembly (learn more about Gibson Assembly below).
 +
<br><br>
 +
We have also added literature-reviewed characterization to the promoter BBa_K2117000, and have planned our own experiments to add further data to the reporter gene BBa_K2117005 that includes this promoter once we have greater lab access. Furthermore, the <a class= "abody" href="https://2020.igem.org/Team:Calgary/Characterize">characterize page </a> also includes in-depth information on our two featured parts: BBa_K3629013 and BBa_K3629016. These two parts are chimeric proteins that have been modelled and enhanced for function in <span style="font-style: italic;class="italic">Y. lipolytica</span>.  
 
         </p>
 
         </p>
  
Line 228: Line 230:
 
<center><img style="padding-bottom:20px;"class="img-fluid"src="https://static.igem.org/mediawiki/2020/f/ff/T--Calgary--validate.jpg">
 
<center><img style="padding-bottom:20px;"class="img-fluid"src="https://static.igem.org/mediawiki/2020/f/ff/T--Calgary--validate.jpg">
  
−
 
−
 
−
 
−
      <h4>VALIDATE</h4>
 
−
<p class="left">Find out how our cellulases functioned in <span style="font-style: italic;class="italic">Y. lipolytica.</span></p></center></a>
 
−
    </div>
 
−
    <div style="padding-top:30px;"class="col-sm-4 break"><a href="https://2020.igem.org/Team:Calgary/Characterize" class="imglink">
 
−
    <center><img style="padding-bottom:20px;"class="img-fluid"src="https://static.igem.org/mediawiki/2020/7/79/T--Calgary--characterize.jpg">
 
  
 
<h4>CHARACTERIZE</h4>
 
<h4>CHARACTERIZE</h4>
Line 242: Line 236:
 
     </div>
 
     </div>
  
 +
<br>
 +
<br>
 +
        <center><h2><b>GIBSON ASSEMBLY</b></h2></center>
 +
<p> Insert brief blurb on how Gibson Assembly works </p>
 
<br>
 
<br>
 
<br>
 
<br>

Revision as of 10:46, 17 October 2020



PARTS OVERVIEW


We have contributed 26 new parts to the registry. All of our parts are included in our Yarrowia lipolytica collection, which includes various basic parts for general genetic circuit construction, devices containing cellulase proteins that have been optimized for high production and secretion, reporter constructs, and other useful devices. Our devices can be connected to each other in a variety of ways using Gibson Assembly (learn more about Gibson Assembly below).

We have also added literature-reviewed characterization to the promoter BBa_K2117000, and have planned our own experiments to add further data to the reporter gene BBa_K2117005 that includes this promoter once we have greater lab access. Furthermore, the characterize page also includes in-depth information on our two featured parts: BBa_K3629013 and BBa_K3629016. These two parts are chimeric proteins that have been modelled and enhanced for function in Y. lipolytica.

PART COLLECTION

Learn more about our Y. lipolytica toolkit which contains parts designed for modular Gibson Assembly.

CHARACTERIZE

See how we plan to test if temperature affects the expression of reporter device BBa_K2117005.



GIBSON ASSEMBLY

Insert brief blurb on how Gibson Assembly works



PARTS LIST

Below is the list of parts we submitted to the registry this year.

<groupparts>iGEM20 Calgary</groupparts>