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+ | <h5 class="modal-title">Dr. Veronique Barthet: The pitfalls of processing...</h5> | ||
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+ | <p>Dr. Veronique Barthet is a Research Scientist and Program Manager for Oilseeds Monitoring and Research at the Canadian Grain Commission's Grain Research Laboratory. Her areas of expertise lie in researching grain quality factors, seed oxidative stability and developing analytical quality measurement methods. She shared with us how the Grain Research Laboratory quantifies the quality of crops, such as canola, in an objective manner. | ||
+ | <br><br>The Canadian Grain Commission relies mostly on spectroscopy and high performance liquid chromatography (HPLC) to determine the chlorophyll content of green seeds. She also told us that chlorophyll a is in the highest abundance in green seed oil, a consideration which would affect our project design. | ||
+ | <br><br>Dr. Barthet’s knowledge of the current method for purifying chlorophyll from canola oil, the acid-activated clay method, also proved to be invaluable to our project development. According to her, the clay used in the purification process is disposed following use, highlighting that creating a re-usable method would bring economic value to the oil processors. The acid-activated clay method is the only purification method that she is aware of meaning that is the only technique we would need to outperform. | ||
+ | <br><br>Finally, Dr. Barthet emphasized that any solution we propose would have to be integrated into current oil processing plants, and would ultimately need to be commercially and economically viable. She believes that as long it is commercially viable, an alternative to acid-activated clays would be welcome in the canola oil processing community.</p> | ||
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Revision as of 05:05, 20 October 2020
HUMAN CENTERED DESIGN
How can we solve problems that affect people?
Mass-supplementation of Vitamin A to deficient regions have been ongoing for decades.However, even with biannual vitamin A supplementation for children and biofortified crops such as golden rice exist, intestinal parasites still play the villain in preventing the vitamin’s assimilation by perforating the intestines. Organizations such as the UN, WHO, and the Bill Gates Foundation couple biannual vitamin supplementation with deworming, but lack of clean water and proper footwear allow these parasites to return and thrive in the intestines. The Oviita system sustainably supplements the consumer with vitamin A and thymol, an anthelmintic agent which is proven to work against worms and their larvae. By doing so, the intestines can recover and maximize absorption of vitamin A in the body.
UNDERSTANDING THE PROBLEM
Who we talked to
To understand the extent of the problem, we talked to people who work directly with the problem.
- Dr. Sanou Dia - Even with VA supplies, intestinal parasites are a big problem. Lack of footwear and clean water cause them to return, and hinder vitamin absorption.
- Dr. Paul E. Mains - There is merit to producing thymol as worms and intestinal parasites may also develop resistance to anthelmintic drugs.
- Lourlin Ugdimay - Something about nutrition deficiencies and how they are distributed, maybe even purga.
- Dr. John Gileard - Parasites are not only problematic in humans, but also livestock. It is important to address this because people from vulnerable areas rely on their livestock for food and income, and there are only 2 main anthelmintic drugs used in the industry, and snps can easily happen.
After our conversations with our HP contacts, we realized that we had to do something about the problem. Since our yeast is meant to be consumed as a nutritional supplement, we need a compound that is a naturally-occuring anthelmintic that is safe for consumption. [insert transition to thymol].
Thymol is a monoterpenoid found in thyme leaves (Thymus vulgaris). It has long been used in traditional medicine for its antioxidant, anti-inflammatory, and antihyperlipidemic properties (Meeran et al. 2017). Although thymol is “Generally Recognized As Safe” (GRAS) by the United States Food and Drug Administration and may be ingested with “negligible toxicity”, extreme doses may induce unwanted side effects such burning pain in the oesophagus, nausea, abdominal pain, vomiting, and dizziness among others (CITE: SCBT Datasheets). This compound is normally administered through essential oils, tablets, or used as a disinfectant by integration in soaps.
INSERT study about thymol pharmacokinetics. And how oil form is absorbed more quickly because thymol is lipophylic, so yarrowia is a good chassis!
Since Y. lipolytica already has the MEP pathway and produces GPP we will only need the addition of two genes: a terpene synthase (TvTPS1) and a cytochrome P450 (CYP71D178).
PART DESIGN
Thoughtful design of genetic constructs
Thymol parts
EXPERIMENTAL DESIGN
Thoughtful design of experiments
[Insert thymol lab workflow graphic here]
Expected Results
For when we get back to the lab
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
FUTURE DIRECTIONS
Next Steps
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
References
Paper 1