Difference between revisions of "Team:Calgary/Characterize"

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           <a href="#experimental-design">Characterizing BBa_K2117000</a>
 
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        <h4>What do the people need? </h4>
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          In order to provide a sustainable, community-based solution, we plan
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<p> <span style="font-style: italic;class="italic">Yarrowia lipolytica</span> is an up and coming yeast species gaining interest in the molecular biology community. Its oleaginous nature and its GRAS status make it a suitable chassis in food and nutrition applications. While there are synthetic biology tools for <span style="font-style: italic;class="italic">Y. lipolytica</span> emerging in the greater scientific community, only a few iGEM teams have worked with this chassis, resulting in a limited number of <span style="font-style: italic;class="italic">Y. lipolytica</span> parts available in the iGEM Registry of Standard Biological Parts. </p>
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          to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous
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          yeast that naturally produces beta-carotene and lipids, to be more
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          robust and resource-efficient. By modifying the yeast to produce
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          cellulase, it can then use common agricultural waste products as an
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          energy source for synthesizing its oil. It can then be eaten as a
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          vitamin A supplement. The yeast strain, while naturally safe and
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          non-pathogenic, will also be genetically modified to include a kill
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          switch for bio-containment, and optimized for oil production.
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<p> While we are providing our <span style="font-style: italic;class="italic">Y. lipolytica</span> <a href="Part_Collection">collection</a> to the registry, two of our parts (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3629013">BBa_K3629013<a/> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3629016>BBa_K3629016</a>) in particular have been modelled and designed for optimal activity in <span style="font-style: italic;class="italic">Y. lipolytica</span>. Once we have greater access to the lab, we plan to characterize these two parts (along with our other parts), and add characterization to an existing <span style="font-style: italic;class="italic">Y. lipolytica</span> promoter in the registry (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2117000">BBa_K2117000<a/>) that is important to our experimental design. Read more about our plans below!
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<p>
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          In order to provide a sustainable, community-based solution, we plan
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          to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous
+
−
          yeast that naturally produces beta-carotene and lipids, to be more
+
−
          robust and resource-efficient. By modifying the yeast to produce
+
−
          cellulase, it can then use common agricultural waste products as an
+
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          energy source for synthesizing its oil. It can then be eaten as a
+
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          vitamin A supplement. The yeast strain, while naturally safe and
+
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          non-pathogenic, will also be genetically modified to include a kill
+
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          switch for bio-containment, and optimized for oil production.
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         </p>
 
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Revision as of 04:40, 25 October 2020



OVERVIEW

Yarrowia lipolytica is an up and coming yeast species gaining interest in the molecular biology community. Its oleaginous nature and its GRAS status make it a suitable chassis in food and nutrition applications. While there are synthetic biology tools for Y. lipolytica emerging in the greater scientific community, only a few iGEM teams have worked with this chassis, resulting in a limited number of Y. lipolytica parts available in the iGEM Registry of Standard Biological Parts.

While we are providing our Y. lipolytica collection to the registry, two of our parts (BBa_K3629013 and Y. lipolytica. Once we have greater access to the lab, we plan to characterize these two parts (along with our other parts), and add characterization to an existing Y. lipolytica promoter in the registry (BBa_K2117000) that is important to our experimental design. Read more about our plans below!


PART DESIGN

Thoughtful design of genetic constructs

In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.


EXPERIMENTAL DESIGN

Thoughtful design of experiments

In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.

FUTURE DIRECTIONS

Next Steps

In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.