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− | <a href="#project-design"> | + | <a href="#project-design">Overview</a> |
<br> | <br> | ||
− | <a href="#part-design"> | + | <a href="#part-design">Engineering Success</a> |
<br> | <br> | ||
− | <a href="#experimental-design"> | + | <a href="#experimental-design">Human Practices</a> |
<br> | <br> | ||
− | <a href="# | + | <a href="#implementation">Proposed Implementation</a> |
<br> | <br> | ||
− | <a href="# | + | <a href="#engagement>Engagement</a> |
<br> | <br> | ||
− | <a href="# | + | <a href="#parts">Parts</a> |
<br> | <br> | ||
− | <a href="# | + | <a href="#modelling">Modelling</a> |
+ | <br> | ||
+ | <a href="#future-directions">Next Year</a> | ||
</div> | </div> | ||
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<div class="intro-body"> | <div class="intro-body"> | ||
<div class = "project-design" id="project-design"> | <div class = "project-design" id="project-design"> | ||
− | <h2> | + | <h2>OVERVIEW</h2> |
− | <h4>What | + | <h4>What we accomplished </h4> |
<p> | <p> | ||
In order to provide a sustainable, community-based solution, we plan | In order to provide a sustainable, community-based solution, we plan | ||
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</p> | </p> | ||
<img src="https://static.igem.org/mediawiki/2019/4/48/T--Calgary--6GIXgel.jpeg"> | <img src="https://static.igem.org/mediawiki/2019/4/48/T--Calgary--6GIXgel.jpeg"> | ||
+ | |||
+ | </div> | ||
<br> | <br> | ||
− | <p> | + | <hr> |
+ | <div class = "part-design" id="part-design"> | ||
+ | <h2>ENGINEERING SUCCESS</h2> | ||
+ | <h4>Cellulase Integration</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Biocontainment</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Omega 3 Fatty Acid Production</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Thymol Production</h4> | ||
+ | <p> | ||
In order to provide a sustainable, community-based solution, we plan | In order to provide a sustainable, community-based solution, we plan | ||
to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
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switch for bio-containment, and optimized for oil production. | switch for bio-containment, and optimized for oil production. | ||
</p> | </p> | ||
− | |||
− | |||
</div> | </div> | ||
<br> | <br> | ||
<hr> | <hr> | ||
− | <div class = " | + | <div class = "experimental-design" id="experimental-design"> |
− | <h2> | + | <h2>HUMAN PRACTICES</h2> |
− | <h4> | + | <h4>Creating a human-centred project</h4> |
<p> | <p> | ||
In order to provide a sustainable, community-based solution, we plan | In order to provide a sustainable, community-based solution, we plan | ||
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switch for bio-containment, and optimized for oil production. | switch for bio-containment, and optimized for oil production. | ||
</p> | </p> | ||
− | </div> | + | |
+ | </div> | ||
<br> | <br> | ||
<hr> | <hr> | ||
− | <div class = " | + | <div class = "implementation" id="implementation"> |
− | <h2> | + | <h2>PROPOSED IMPLEMENTATION</h2> |
− | <h4> | + | <h4>Bioreactor</h4> |
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Randle's Cell Testing Device</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Entrepreneurship</h4> | ||
<p> | <p> | ||
In order to provide a sustainable, community-based solution, we plan | In order to provide a sustainable, community-based solution, we plan | ||
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switch for bio-containment, and optimized for oil production. | switch for bio-containment, and optimized for oil production. | ||
</p> | </p> | ||
− | |||
− | <div | + | </div> |
− | + | <br> | |
− | + | <hr> | |
− | + | <div class = "engagement" id="engagement"> | |
− | + | <h2>ENGAGEMENT</h2> | |
− | + | <h4>Education</h4> | |
− | < | + | <p> |
− | + | In order to provide a sustainable, community-based solution, we plan | |
− | + | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | |
− | + | yeast that naturally produces beta-carotene and lipids, to be more | |
− | + | robust and resource-efficient. By modifying the yeast to produce | |
− | + | cellulase, it can then use common agricultural waste products as an | |
− | + | energy source for synthesizing its oil. It can then be eaten as a | |
− | + | vitamin A supplement. The yeast strain, while naturally safe and | |
− | + | non-pathogenic, will also be genetically modified to include a kill | |
− | + | switch for bio-containment, and optimized for oil production. | |
− | + | </p> | |
− | + | <h4>Collaborations</h4> | |
− | + | <p> | |
− | + | In order to provide a sustainable, community-based solution, we plan | |
− | + | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | |
− | + | yeast that naturally produces beta-carotene and lipids, to be more | |
− | + | robust and resource-efficient. By modifying the yeast to produce | |
− | + | cellulase, it can then use common agricultural waste products as an | |
− | + | energy source for synthesizing its oil. It can then be eaten as a | |
− | + | vitamin A supplement. The yeast strain, while naturally safe and | |
− | + | non-pathogenic, will also be genetically modified to include a kill | |
− | + | switch for bio-containment, and optimized for oil production. | |
− | + | </p> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
+ | </div> | ||
+ | <br> | ||
+ | <hr> | ||
+ | <div class = "parts" id="parts"> | ||
+ | <h2>PARTS</h2> | ||
+ | <h4>Characterize</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Validate</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | </div> | ||
+ | <br> | ||
+ | <hr> | ||
+ | <div class = "modelling" id="modelling"> | ||
+ | <h2>MODELLING</h2> | ||
+ | <h4>Bellatrix</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Protein Modelling</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>GausHaus</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Metabolic Flux</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
+ | <h4>Measurement</h4> | ||
+ | <p> | ||
+ | In order to provide a sustainable, community-based solution, we plan | ||
+ | to genetically modify <i>Rhodosporidium toruloides</i>, an oleaginous | ||
+ | yeast that naturally produces beta-carotene and lipids, to be more | ||
+ | robust and resource-efficient. By modifying the yeast to produce | ||
+ | cellulase, it can then use common agricultural waste products as an | ||
+ | energy source for synthesizing its oil. It can then be eaten as a | ||
+ | vitamin A supplement. The yeast strain, while naturally safe and | ||
+ | non-pathogenic, will also be genetically modified to include a kill | ||
+ | switch for bio-containment, and optimized for oil production. | ||
+ | </p> | ||
− | + | </div> | |
− | + | <br> | |
− | + | ||
− | </div> < | + | |
<hr> | <hr> | ||
<div class = "Future Directions" id="future-directions"> | <div class = "Future Directions" id="future-directions"> | ||
− | <h2> | + | <h2>NEXT YEAR</h2> |
− | <h4> | + | <h4>Our goals for next year</h4> |
<p> | <p> | ||
In order to provide a sustainable, community-based solution, we plan | In order to provide a sustainable, community-based solution, we plan |
Revision as of 18:28, 8 October 2020
OVERVIEW
What we accomplished
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
ENGINEERING SUCCESS
Cellulase Integration
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Biocontainment
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Omega 3 Fatty Acid Production
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Thymol Production
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
HUMAN PRACTICES
Creating a human-centred project
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
PROPOSED IMPLEMENTATION
Bioreactor
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Randle's Cell Testing Device
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Entrepreneurship
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
ENGAGEMENT
Education
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Collaborations
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
PARTS
Characterize
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Validate
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
MODELLING
Bellatrix
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Protein Modelling
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
GausHaus
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Metabolic Flux
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
Measurement
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.
NEXT YEAR
Our goals for next year
In order to provide a sustainable, community-based solution, we plan to genetically modify Rhodosporidium toruloides, an oleaginous yeast that naturally produces beta-carotene and lipids, to be more robust and resource-efficient. By modifying the yeast to produce cellulase, it can then use common agricultural waste products as an energy source for synthesizing its oil. It can then be eaten as a vitamin A supplement. The yeast strain, while naturally safe and non-pathogenic, will also be genetically modified to include a kill switch for bio-containment, and optimized for oil production.