Team:GunnVistaPingry US
Odigos: An Improved CRISPR-Cas9 Effective Guide RNA Predictor
By GunnVistaPingry_US, a high-school track iGEM Team
Odigos: An Improved CRISPR-Cas9 Effective Guide RNA Predictor
By GunnVistaPingry_US, a high-school track iGEM Team
Abstract
CRISPRi is a powerful tool for modulating gene expression in human cells. By designing a gRNA homologous to the target gene of interest, one can achieve targeted knockdown of the specific gene of interest. However, with current methodologies, one has to screen multiple gRNA sequences for efficient targeting while minimizing off-target effects. We present a prediction model for identifying the best gRNA sequence for efficient gene targeting in human cells. Starting with experimental data from knocking down specific genes using several gRNAs in iPS cells, we leverage machine learning to inform better selection of the gRNA. Our tool will be invaluable for designing gene targeting gRNAs and will reveal underlying biochemical principles governing CRISPR efficiency.
