Check and optimize your sequences on Twist Bioscience’s online ordering platform. This tool analyzes your sequences and will highlight any potential issues. Follow the detailed instructions below to check whether your sequences are ready for synthesis.

Finally

Receive your Free Twist Gene Fragments

Our team will reply within 1 working day to obtain your shipping information. Our scientists will kick off the synthesis of your DNA sequences, and ship them to you within 6 - 9 business days*.

*Turnaround time applies to Europe and USA and are estimates. Teams outside these regions may be subject to longer delivery times. Our support team will be able to provide an accurate estimated delivery time.

The deadline for ordering FREE DNA is October 13th

A Step-by-Step approach to getting your FREE FRAGMENTS

Step 1:
Sign up for Twist’s online ordering platform here. For teams logging in from China - Please refresh the page if the link does not work

Step 2:
On the “What Can Twist Build For You?” page, select “Genes”. Name your project and click “Start New Project”. On the next page click “Continue” to get started.

Step 3:
Select “Nucleotide Sequence” and “Manual Import” to go to the sequence input screen or upload your sequences directly.

Step 4:
Paste in your sequences and click “Continue” to upload them into the sequence checking tool. The tool will begin analyzing your sequences immediately.

Step 5:
If your sequences show a green circle, they are ready to go - skip to Step 7. If they show a black circle, there is an issue - proceed to Step 6.

Step 6:
Optimize your sequences with our sequence optimization tool. Contact the Twist Support Team (customersupport@twistbioscience.com), or click the link to the chat box on our online ordering platform if you have questions about the sequence optimization process. Make sure to tell us you are participating in iGEM so we can offer the best possible support.

Step 7:
Once you have optimized your sequences, save them to update your sequence list

Step 8:
Select all of your sequences and click “export table”.

Step 9:
Send this CSV file to igemquestions@twistbioscience.com in an email titled “iGEM free DNA for [your team name] from [country]”.

REMEMBER: DON’T CLICK “CHECKOUT” or you will not be able to access your free gene fragments.

WHEN YOU RECEIVE YOUR DNA

Once you receive your synthetic DNA, follow these easy instructions to use your products: READ INSTRUCTIONS ⇗

QUESTIONS?

Have any questions about your order? Please contact: igemquestions@twistbioscience.com

TWIST IS TRANSFORMING THE SAMPLE SUBMISSION PROCESS

This year iGEM is partnering with Twist Bioscience so teams no longer need to send physical DNA samples to the Registry. Instead, teams will submit their sequences and accompanying data and Twist will handle the synthesis of your submitted parts.

To learn more, visit iGEM’s sample submission page.

At Twist Bioscience, we work in service of customers who are changing the world for the better. In fields such as medicine, agriculture, industrial chemicals, and data storage, Twist’s customers are using our synthetic DNA tools to develop ways to live healthier, more sustainable lives, and improve the overall health and sustainability of the planet.

Twist Bioscience synthesizes DNA from scratch utilizing a proprietary semiconductor-based synthetic DNA manufacturing process. This process, known as “writing” DNA, features a high-throughput silicon platform capable of producing a diverse offering of DNA-based products and solutions. Twist Bioscience’s DNA writing technology uses the same chemical and molecular biology reagents as the conventional approach to synthesizing DNA, but miniaturizes the reaction volumes by orders of magnitude. By synthesizing DNA on silicon instead of on traditional 96-well plastic plates, this platform overcomes the current inefficiencies of synthetic DNA production and enables cost-effective, rapid, high-quality, and high-throughput DNA synthesis to accelerate research and development.

Twist’s synthetic DNA can be used to power a myriad of applications, and Twist has built a number of DNA-based product portfolios to enable researchers to reimagine and engineer biology to find answers to some of the biggest challenges we face on earth.

Popular Product Offerings

Gene Fragments ⇗

Screen Less, Discover More

dsDNA
0.3 kb - 1.8 kb
100 ng - 1 ug per fragment
1:3,000 bp avg error rate
From 6-9 business days turnaround

Clonal Genes ⇗

Friends Don't Let Friends Clone

dsDNA cloned into vector
0.3 - 5 kb
50 ng - 2 μg
NGS verified perfect sequences
Several prep scales available
From 11 business days turnaround

Oligo Pools ⇗

Build Better High Throughput Screens

Pools of ssDNA up to 300nt
Unlimited unique sequences per pool
>0.2 fmol per oligo
Starting from 5 business days turnaround

Resources

Customer Profiles:

Case study featuring Vanderbilt focused on Genes

Webinar featuring Stephen Pettitt, PhD, Institute of Cancer Research, focused on Oligo Pools

Technical Note based on the work by Manfred Reetz, PhD, Max-Planck-Institut, focused on Libraries

Webinar featuring Brendan Blumenstiel, Broad Institute, focused on NGS Target Enrichment

Product Information

Gene Fragments Product Sheet

Gene Fragment Cloning Guidelines

Clonal Genes Product Sheet

Twist Vectors for Clonal Genes

Oligo Pools Product Sheet

Site Saturation Libraries Product Sheet

Combinatorial Libraries Product Sheet

Twist Human Core Exome Product Sheet

Twist Custom Panel Product Sheet

DNA Resuspension Guidelines

Twist Bioscience is a Platinum Partner of the 2020 iGEM Competition

As a non-profit organization, we count on the support of our partners and sponsors to help us continue to provide this unique and valuable experience to students worldwide. Learn more about Sponsorship at iGEM.

Sponsors/TwistBioscience

Twist Bioscience

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